c-pace electrical pulse stimulation (eps) apparatus  (IonOptix)

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Mechanical overload-induced muscle-derived extracellular vesicles promote adipose tissue lipolysis

doi: 10.1096/fj.202100242R

Figure Lengend Snippet: Human skeletal muscle down-regulate miR-1 associated with release of EV containing miR-1 in response to a hypertrophic stimulus. A, Decreased in miR-1 expression 30 min after a bout of resistance exercise compared to pre-exercise. B, Increased miR-1 abundance from EVs isolated from plasma 30 min post-exercise compared to pre-exercise. C, Representative images of human myotubes with or without electrical pulse stimulation (EPS). Myotubes were stained with a pan myosin antibody to accurately measure myotube diameter. D, Increased human myotube diameter 24 h post-EPS. E, Decreased miR-1 expression in primary human myotubes 24 h post-EPS and in (F) Increased EVs miR-1 abundance from culture media of EPS treated myotubes. G, No change in EV concentration isolated from culture media of myotubes with or without EPS. Data are expressed as mean ± SE (n = 10 for A-B; n = 3 for D-F; n = 7 for G) with significance (P < .05) denoted by an asterisk compared to respective sham

Article Snippet: Human MPCs were differentiated for 5 days with fresh media added prior to electrical pulse stimulation (EPS, Ionoptix C-Pace EP) for 8 h at 12 V, 1 Hz, 2 ms, followed by a 16 h rest period.

Techniques: Expressing, Isolation, Clinical Proteomics, Staining, Concentration Assay

Journal: American Journal of Physiology - Cell Physiology

Article Title: Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism

doi: 10.1152/ajpcell.00540.2019

Figure Lengend Snippet: Response to electrical pulse stimulation (EPS) in L6, C2C12, and human primary myotubes. A : mRNA levels of myosin and actin isoforms. B : representative images of myotubes during proliferation and after complete differentiation. Staining for desmin and myosin was performed using specific antibodies as described in methods . Representative images are shown for each staining. C : protein content per well measured by bicinchoninic acid assay. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 11 replicates from 11 independent donors, C2C12: n = 7 independent experiments, L6: n = 6 independent experiments, Kruskal-Wallis test with Dunn’s multiple comparison. D–F : mRNA levels of the GTPase-activating protein for Rab family protein TBC1D1 , Ca 2+ /calmodulin-dependent protein kinase II ( CAMK2A ), and RAC1 . G : electrical pulse stimulation-induced glucose uptake measured using 2-[1,2- 3 H]deoxy- d -glucose. Mean ± SE and individual data points: n totals are as follows: HSMC: n = 7 replicates from 7 independent donors, C2C12: n = 6 independent experiments, L6: n = 7 independent experiments, one-way ANOVA with Tukey’s multiple comparison. H and I : mRNA expression of exercise-responsive genes measured by quantitative PCR. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 5 replicates from 5 independent donors, C2C12: n = 6 independent experiments, L6: n = 6 independent experiments, two-way ANOVA with Fisher’s least-significant difference comparison. # P < 0.05, significant response to EPS. * P < 0.05. HSMC, human skeletal muscle cells.

Article Snippet: Differentiated myotubes were subjected to electrical pulse stimulation (EPS; C-Pace EP; Ionoptix, MA) at 40 V, 2 ms, 1 Hz during 3 h in low-glucose DMEM.

Techniques: Staining, Acid Assay, Whisker Assay, Comparison, Expressing, Real-time Polymerase Chain Reaction